You could, for example, create three versions of the same newsletter, each with a different subject line, and send it to 1,000 people on your database; after a few hours, you ll be able to identify which subject line led to the best open rate, and then send a message with the winning subject line to the remainder of your list.

http://merritt.instructure.com/eportfolios/730/Atarax_25_Mg_Para_Dormir__Dr_ReddyS__Antidepressants_
http://reach.instructure.com/eportfolios/836/Montair_Chewable_Uz_Tablets_500Mg__Cipla__Asthma_
http://aste.instructure.com/eportfolios/1030/What_Is_Prosoma_500__Centurion_Laboratories__Pain_Management


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